Purification of xylanase produced by Bacillus pumilus
نویسندگان
چکیده
منابع مشابه
Partial Purification and Characterization of Bacillus Pumilus Xylanase from Soil Source
Hydrolysis of xylan, the chief type of hemicellulose is achieved by endo-1, 4-β-xylanase and βxylosidase among other such enzyme complex. These enzymes are mainly produced by fungi, bacteria, etc. Xylanase finds applications in animal feed, manufacture of bread, beverages, textiles, bleaching cellulose pulp, ethanol and xylitol production. Xylanase depolymerizes xylan molecules into xylose unit...
متن کاملOptimization of the Cellulase Free Xylanase Production by Immobilized Bacillus Pumilus
Background: The extracellular xylanase secreted by microorganisms is a hydrolytic enzyme, which arbitrarily cleaves the β-1, 4 backbone of the polysaccharide xylan; an enzyme used in the food processing, bio-pulping and bio-bleaching. The commercial production of the xylanase is limited because of a higher cost involvement, which can be overcome by the cost-effective production...
متن کاملOne-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash
The present study describes the one-step purification and characterization of an extracellular cellulase-free xylanase from a newly isolated alkalophilic and moderately thermophilic strain of Bacillus subtilis ASH. Xylanase was purified to homogeneity by 10.5-fold with ~43% recovery using ion-exchange chromatography through CM-Sephadex C-50. The purified enzyme revealed a single band on SDS-PAG...
متن کاملPurification and characterization of a new xylanase (xylanase B) produced by Streptomyces lividans 66.
A new extracellular xylanase produced by Streptomyces lividans 66 was isolated from a genetically engineered clone of that strain. This enzyme, named xylanase B, has an Mr of 31,000 and acts specifically on xylan as an endo-type xylanase producing short-chain xylo-oligosaccharides. The activity is optimal at pH 6.5 and at a temperature of 55 degrees C, which is similar to that of the previously...
متن کاملApplication of Statistical Design to Optimize Culture Medium for Xylanase Production by Bacillus pumilus AB-1
234 Application of Statistical Design to Optimize Culture Medium for Xylanase Production by Bacillus pumilus AB-1 Snehal Ingale, Sonam Kalyani 1 and Urvish Chhaya Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology & Allied Sciences, New Vallabh Vidyanagar-388120, Gujarat, India Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar-388120, Gujar...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of the National Science Foundation of Sri Lanka
سال: 2014
ISSN: 2362-0161,1391-4588
DOI: 10.4038/jnsfsr.v42i4.7736